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海洋科学  2012 

Embryonic development of and disease of infusorium in Perinereis aibuhitensis
双齿围沙蚕的胚胎发育与纤毛虫病害

Keywords: Perinereis aibuhitensis,embryonic development,pathgeny
双齿围沙蚕(Perinereis
,),胚胎发育,病害

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Abstract:

The mature Perinereis aibuhitensis came from the semi-artificial cultured populations in 2010.During July and August,2011,some of the cultured populations was matured into heteronereis.Though the heteronereis can reproduce under artificial conditions when both male and female individuals(30~50) are pooled into one palstic basket,we used artifical fertilizing methods in order to obtain synchronously ontogenitic embryoes,then to faciliate the following larvae-culturing regimes.After disinfection,the fertilizing eggs were transferred into cement pond for middle culture.When the embryo developed into the ontogentic stage of 3-chaetae nectochaete on day 3 post fertilization,the larvae were collected from cement pond,and put into mud pond for last cultivation.The early ontogeny of P.aibuhitensis was observed under Olympus –cx31 microscope.The typical characteristic images were taken using charge-coupled device(CCD) and SONY H-50 camera.There were some phenomena different from previous reports,including numbers of oil globules vs embryonic development,temperature vs embryonic development.One of pathogenic ciliate species emerged during the early development and resulted in mass death of embryo and larvae of P.aibuhitensis.

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