The variation analysis of mtDNA D-loop sequence of Cheilinus undulatus
波纹唇鱼mtDNA D-loop 序列变异分析

The aim of this paper was to study the variations in the mtDNA D-loop sequences of Cheilinus undulatus populations. Twenty-five individuals of C. undulatus were collected from the offshore area of Hainan province. One thousand and four hundreds bp nucleotide sequences of mtDNA D-loop region were obtained using PCR and cloning techniques. 66 variation sites including 0 deletion, 4 inserts, 59 transition sites, 2 transversion sites and 1 transition-transversion sites were observed using ClustalX and MEGA5.0 softwares. The pairwise genetic distances of these 25 individuals were calculated using the MEGA5.0 software and based on that, the NJ phylogenetic trees was contracted for these fish populations. Analysis of this fish populations using the DNASP software showed that they had a polymorphic sites (S) of 62, a nucleotide diversity (Pi) of 0.00606 and an average number of nucleotide differences (K) of 6.907, respectively. Overall, there were no significant variations between mtDNA D-loops of C. undulatus populations.