Synergistic degradation of 2-chloronitrobenzene by two strains of bacteria
两株细菌对邻氯硝基苯的协同降解

Under the conditions of pure and simulated water body culture, two strains of indigenous bacteria Acinetobacter calcoaceticus and Pseudomonas putida, which are able to effectively degrade chlorobenzene and nitrobenzene respectively, were used to study synergistic degradation of 2-chloronitrobenzene. Results showed that the order of capacity to degrade 2-chloronitrobenzene was the mixture of two strains>Pseudomonas putida>Acinetobacter calcoaceticus. Under pure culture conditions, 94.5% of 2-chloronitrobenzene was degraded by the mixture of strains after 6 d of incubation. The degradation of 2-chloronitrobenzene was accompanied by the release of NO-2 and Cl-. The release of NO-2 was earlier than that of Cl-, but the total net released Cl- was more than that of NO-2. The addition of glucose significantly accelerated the growth of bacterial cells and decreased the accumulation of NO-2, but failed to promote the degradation of 2-chloronitrobenzene. In the conditions of simulated water body culture, the mixture of the strains played the main role in the removal of 2-chloronitrobenzene in the aqueous phase. The sludge, pebbles and organisms attached to the surface of pebbles obviously promoted the removal of 2-chloronitrobenzene. After 8 d of incubation, the removal rate of 2-chloronitrobenzene (92.5%) in the simulated system with sludge was larger than that in the simulated system with pebbles (85.5%). The findings of this research can provide a new pathway for the removal of 2-chloronitrobenzene in the environment.