Isolation and identification of a filamentous cyanobacterium and its tolerance for crude oil
一株丝状蓝藻的分离鉴定及其对原油耐受性能的研究

A filamentous cyanobacterium strain GH1 was isolated from oil-contaminated water collected from Huangpu Port, and was identified as Oscillatoriales by using morphological and molecular methods. The tolerance of crude oil of the strain GH1 was studied from two aspects including its growth and the activity of antioxidant enzyme. The chlorophyll of Oscillatoriales GH1 in the cultivation with crude oil was increased more rapid than the control, and could achieve the stable breading period in the 7th day. The contents of soluble protein were increased first but decreased afterwards during the 20d cultivation. The concentrations of soluble protein in the samples under crude oil condition were always significantly higher than those in control group. Both concentrations of soluble protein and chlorophyll were promoted by 0~1% (v:v) crude oil. For the antioxidant enzyme system, the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly higher than the control group in the initial culture stage with crude oil cultivation. The SOD and CAT decreased but peroxidase (POD) keep increasing with time. All these three antioxidant enzymes were promoted by low concentration of crude oil. High concentration of crude oil could inhibit the activity of SOD and CAT, while the activity of POD still increased with the increasing of crude oil. There should have a complementary effect between SOD, CAT and POD to resist the toxic substances in crude oil. This complementary cooperation of the three enzymes would be the main mechanism for Oscillatoriales GH1 to tolerate crude oil.