AN IN VITRO SCE DETECTING SYSTEM OF CHROMOSOMES IN M. ALBUS AND ITS USE IN THE ASSAY OF MUTAGENICITY OF DETERGENTS
黄鳝染色体体外SCE检测系统的建立及对两种清洗剂的检测

The sensitivity of sister chrom atid exchanges (SCEs) of blood lymph-ocytes of M. albus was tested with different dosages of mtomycin C (MMC) and cyclophosphamide (CP), u sing SCE analysis technique. The results showed that the spontaneous frequency of SCEs was quite low (0.527 SCEs/M. P). However, the frequen cies of SCEs induced with MMC or CP increased by 1.24 to 23.37 times and 2.50 to 6.5 times respective-ly, comparing to the control group. The SCEs induced by MMC and CP in M. albus were much more sensitive than those in other animals. The technique was used to assay the mutagenicity of detergents and it was found that a cleaner and a washing powder at concentrations of 0.2 to 0.6 ug/m could causa significant iacrease in frequency of SCEs.