Construction of Electrochemiluminescence System for Harmful Algae Detection
赤潮藻电致化学发光分子探针检测系统的构建

To develop a new technique, Electrochemiluminescence-Molecular Probe (ECL-MP), for harmful algae detection, a highly sensitive electrochemiluminescence (ECL) detection system was set up based on the principle of ECL and related literature. The optimization tests were carried out, including the impact of voltage and current, the concentration of tripropylamine (TPrA) and the pH of phosphate buffer (PBS). The determination limit of Ru(bpy)3Cl2·6H2O was 10-11 mol·L-1 and the detection range was from 10-9-10-5 mol·L-1as well as the detection amount of substance was in the range from 0.4 pmol-4 nmol in the optimal reaction conditions with voltage of 1.0 V, current of 1.0 mA, TPrA concentration of 1.5 mol·L-1 and pH of 7.4. It was proved by facts that this ECL analyzer was stable and highly sensitive, which was helpful in establishment of ECL-MP.