Recombinant hPR Gene Yeast for Assessing in Drinking Water
应用重组孕激素基因酵母测定饮用水中内分泌干扰物的方法

A bioassay method using recombinant human progesterone receptor (hPR) gene yeast to evaluate the environmental endocrine disrupter effects was introduced. This method was used to determine the inhibition of hPR activity in pollutants emitted from a water works in the south China. The result showed that the recombinant gene yeast had steroid specificity and dose-respond curve for progesterone with EC50 value of 0.5 nmol/L; The estrogenic chemicals pentachlorophenol and p-nonylphenol inhibited the activity of hPR in the yeast which had IC50 values of 2.4 micromol/L and 3.7 micromol/L, respectively. The results indicate that the recombinant progesterone gene yeast assay technique is an efficient and rapid method for screening and quantitatively analyzing the endocrine disrupters which can be the inhibitor of hPR activity. Combined with the solid-phase extraction (SPE) procedure, the yeast assay showed an obvious inhibition of hPR activity for water works sample and the inhibition rates were more than 58 %. Therefore, it is reliable to use the recombinant hPR gene yeast for assessing the environmental endocrine disrupters.