Effects of Copper (Cu2+ ) and Cu-EDTA Complex on the Induction of HSP70 in the Fish Brain
Cu2+和Cu-EDTA对鲫鱼脑组织应激蛋白HSP70诱导的影响

The effects of copper (Cu2+) and Cu-EDTA complex on the induction of HSP70 in the fish brain were studied.After the fish (Carassius auratus) were exposed to the experimental concentrations for forty days , the SDS-PAGE and Western Blotting techniques were used to detect the expression level of HSP70 in the fish brain.The results showed that Cu2+ and Cu-EDTA complex can induce the expression of HSP70 significantly.Comparing with the test groups exposed to Cu2+,the Cu-EDTA complex had changed the bioavailability and decreased the toxicity of the heavy metal.It was found that the fish exposed to the concentration of Cu2+ which below the national fishery water quality standard of 0.01mg/L , also had a significant increase in the expression level of HSP70. Therefore, HSP70 as an environmental biomarker can be a more sensitive and rapid indicator than conventional end points in the aquatic ecosystem.