Flow Injection Biosensor Based on the Immobilized AChE
基于固定化AChE的流动注射型酶传感器研究

A biosensor based on the flow injection system was constructed with the immobilized AChE from Scomberomorus niphonius(Curier) as identification element and a pH electrode as transducer.When phosphate buffer was used as carrier liquid,a good reproducibility(RSD=1.427%,n=10) of the biosensor response was obtained after the substrate was injected repetitively.After an incubation time of 20min,the calibration graph to methyl-parathion is linear(r=0.998?6)when its concentration ranges from 4.29×10-10 mol·L-1 to 4.29×10-8 mol·L-1,and the detection limit is 1.3×10-10 mol·L-1.However,the sensitivity of this biosensor to methyl-parathion when using clean seawater as carrier liquid is not as good as that using phosphate buffer.But after preoxidation of methyl-parathion with NaClO as oxidant,the detection limit of the biosensor to methyl-parathion in seawater can be improve to 2.16×10-7 mol·L-1.