THE IMMUNE PROTECTION EFFECTS OF TWO POLYSACCHARIDES AS THE ADJUVANTS OF INACTIVATED EDWARDSIELLA TARDA VACCINE IN TURBOT SCOPHTHALMUS MAXIMUS
两种多糖作为迟缓爱德华氏菌（Edwardsiella tarda）灭活疫苗佐剂对大菱鲆（Scophthalmus maximus）的免疫保护效果

The immune protection effects of two polysaccharides as the adjuvants of inactivated Edwardsiella tarda vaccine in turbot were studied in this research. The vaccines were prepared with two polysaccharides, astragalus polysaccharides (APS) and glucan (Glu) were added to the inactivated E. tarda vaccine separately. Turbots Scophthalmus maximus were inoculated by intraperitoneal (i.p.) injection. The lysozyme activity (Lyz), superoxide dismutase activity (SOD), and antibody titer in the sera were determined after 28 day post-vaccination; meanwhile, the relative percentage survival (RPS) in each group was compared. The results indicated that the addition of polysaccharide adjuvants in the inactivated vaccine could enhance the immune functions to the vaccinated turbots. The Lyz, SOD, and the antibody titer in the sera of the turbots inoculated with the mixtures of inactivated vaccine and each polysaccharide adjuvant were significantly increased (P<0.05). The RPSin the vaccination group with the mixture of inactivated vaccine and 2.5mg/ml APS and the vaccination group with the mixture of inactivated vaccine and 5mg/ml Glu have reached to the best protection effects at (78.7±1.3)% and (64.0±8.9)%, respectively. The SOD, Lyz, and the antibody titer in the best RPSgroups with the two polysaccharides also reached higher levels than those in the other groups did.