STUDY ON THE DEVELOPMENT OF SNP MARKERS IN SCOPHTHALMUS MAXIMUS USING HIGH-RESOLUTION MELTING (HRM)
应用高分辨率熔解曲线(HRM)开发大菱鲆(Scophthalmus maximus)SNP标记的研究

The experiment mines Scophthalmus maximus 12428 EST sequence from NCBI database. Putative SNP were detected from assembled contigs using Vector NTI Advance 11. SNP genotyping was performed using high-resolution melting to different character populations. Primers and probes were designed using Primer5 and Oligo7, respectively. The results show that, More than 160 putative SNP were found from 522 contigs; 56 putative SNP were selected for primer design and for SNP marker development, 37 pairs of primer were successfully amplified, including 45 putative SNP site, the success rate is 66.1%; Probes which can hybrid with Putative SNP sites have 13, hybridization Rate is 28.9%; 8 sites of hybridization sites displayed polymorphisms in different character populations, accounting for 61.5% of the hybrid sites. There are 6 transition sites, namely the G-A and C-T are 3 of each. There are 2 transversion sites, namely the T-G and A-T are 1 of each