Novel progress in separation and purification technology of nattokinase
纳豆激酶集成化分离技术

The progress in separation and purification technology of nattokinase is reviewed. Based on the analyze of normal purification technology, the novel technologies about the integration of bioseparation processes are introduced, such as aqueous two-phase partitioning integrated with affinity ligand, expanded bed adsorption, and mixed-mode adsorption technology. In general, integration of bioseparation processes provides a good way to improve and optimize the whole separation process of nattokinase and other proteins.