Research on N-fixing property of bacteria with N-fixation and dissolving P function
兼具固氮、解磷功能菌株固氮特性的研究

The N-fixing property of 9 bacteria with N-fixation and dissolving P function was studied under the conditions of non-nitrogen, different P resources with sufficient nitrogen and their mixed incubation. The results show that there are different N-fixation activities under the non-nitrogen condition, however, the volume of N-fixation has more diversity. The bacteria with the highest N-fixation amount is N5-1, reaching 55.4mg/kg and 3.1 times as much as the lowest "K10-2". When taking Ca 3(PO 4) 2 as P resource, "K10-2" and "N17" still have an active N-fixing ability under sufficient nitrogen supplied, the increasing range of total nitrogen in the culture solution gets to 55.2% after 3 days incubation. The N-fixation content of "N11", "N12", "P14-2" also maintain on a higher level, the total nitrogen increases by 30% or so. The total nitrogen of "K16" in the culture solution has a little increase. "N5-1", "N13-1" and "K3" result in the volatilization loss of nitrogen. These processes will be reserved when taking the ground phosphate rock as the P-resource. Compared to the single culture, the mixed incubation on Ca 3(PO 4) 2 medium can effectively decrease N-loss of "N5-1", "N13-1", and "K3".