Subpopulation differentiation of Anthoxanthum alpinum (Poaceae) along an altitudinal gradient detected by random amplified polymorphic DNA
应用RAPD技术研究阿尔卑斯山黄花茅居群内的遗传分化

Random amplified polymorphic DNA (RAPD) phenotypes generated by 15 primers were scored in 5 subpopulatious of Anthoxanthum alpinum along an altitudinal gradient. Although few subpopulation-specific markers were found, a significant population differentiation was revealed by the use of principal factor analysis and Ward' s cluster analysis. The result was also confirmed by the significant positive correlation when comparing beth the matrix of similarity between the individu- als within subpopulations and the matrix among subpopulations, as well as the matrix of distances and the differences of altitude between the subpopulations (Mantel test). This study suggests that the differences of altitude between subpopulation sites may result in the differences of phenologicalperiod for flowering and growth which restrict gene flow.