Molecular Pre-identification of Interspecies Hybrids in Prunus
樱桃属种间杂种的早期分子鉴定

Incompatibility of interspecies cross between sweet cherry and Cerasus tomentosa was overcome by successive hand pollination, and the new germplasm G3 was obtained by using embryo culture technology to overcome the embryo abortion of distance hybridization. The G3 S-genotype was identified in seedling using AS-PCR, and the self-incompatibility gene of the parent was used as the molecular marker to validate the pedigree of hybrid G3. The RAPD analysis further demonstrated that G3 share characteristic RAPD fragments of both parents. This result suggested that AS-PCR was an effective and credible method to identify the progeny of interspecies hybrid in Prunus. And the cross could be a promising way to enhance the genetic background. DNA molecular marker realized the identification and pre-selection in the seedling stage. So the developing breeding program would be accelerated by the new way.