Cloning and Expression of a Gene Encoding Shortened Cecropin A-Melittin Hybrid in E.coli
天蚕抗菌肽Ａ与蜂毒素杂合肽基因的合成及在大肠杆菌中的克隆与表达 Cloning and Expression of a Gene Encoding Shortened Cecropin A-Melittin Hybrid in E.coli

According to the studies of David Andreu and other scientists,we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid.This hybrid consists of CA(1-7) and M(5-12).The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI.A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).