利用基因芯片对一高保守基因进行肿瘤相关分析 Tumor Relevance Analysis of A Highly Conserved Gene by Using Gene Microarray Hybridization

本文对大规模人类cDNA 测序过程中获得的一条高保守基因进行了初步功能研究，生物信息学研究发现该基因在人类、小鼠、果蝇、拟南芥和裂殖酵母中都有很高的保守性，其他分析预测该基因可能具有肿瘤相关性。RT-PCR分析表明，该基因在成人和胎儿组织中广谱表达。利用基因芯片分析该基因在7例肝癌、5例胰腺癌、2例喉癌和2例肺癌中表达情况，结果证实了该基因的肿瘤相关性，并且提示该基因在不同肿瘤类型中可能处于不同的地位。 Abstract:Preliminary function research of a highly conserved human gene,which was cloned from human fetal cDNA library during large-scale cDNA sequencing,is illustrated in this article.Bioinformatics analysis indicates that this gene is highly conserved in human、mouse、fruit fly、thaliana and fission yeast.Other bioinformatics analysis implies its relevance with tumors.RT-PCR analysis shows its wide-ranging expression patterns.Its expression in 16 cancer cases(including 7 liver cancer cases、5 pancreas cancer cases、2 larynx cancer cases and 2 lung cancer cases) is studied by using gene microarray analysis.The result shows its relevance with tumors and implies it may have different status in different classification of tumors.