Construction and analysis of cDNA library of Yunnan Yuanjiang O. rifupogon leaf
元江普通野生稻叶片cDNA文库的构建及部分基因片段分析

The cDNA library of Yuanjiang Oryza rifupongon leaf was constructed by using SMARTTM technology. The titers of the non-amplified library and the amplified library were 1.1×106 pfu/mL and 3.98×107 pfu/mL, respectively. The recombi-nation rate was more than 91%. The DNA sequence length of the most cDNAs in the library was between 500-2 000 bp. Some cDNAs chosen by random were sequenced. After BLAST analysis of some cDNAs, their possible function were pre-dicted. It is found that these cDNAs show 98% similarity to Oryza sativa japonica in the NCBI database. These provided a base for further study on the structure and function of these cDNAs and evolutionary process of Yuanjiang Oryza rifupongon .