The Correlation Between the Number of RAPD-loci and the Reliability of the Information on Genetic Variation in Molecular Phylogenetic Studies
分子系统学研究中分子位点数与遗传差异信息可靠性的关系 The Correlation Between the Number of RAPD-loci and theReliability of the Information on Genetic Variation in MolecularPhylogenetic Studies

We studied the correlation between the number of RAPD-loci investigated and the reliability of the information on genetic variation analysis using quite different two-kind materials, silkworms (including 59 geographic varieties of Bombyx mori belonging to different ecotype, as well as Bombyx mandarina M ) and mulberries (including 12 species and 2 varieties) The results showed as follows: (1) There was obvious correlation between the numbers of RAPD-loci investigated and the reliability of the information on genetic variation analysis (2) The data were not suitable for the genetic variation analysis when the number of RAPD-loci investigated was smaller than 20 The more RAPD loci were analyzed, the more accurate genetic diversity would be identified when the number of the locus was varied from 20 to 70 Furthermore, the stable information to be available is shown when the number exceeded 70 (3) The similar regularities mentioned above were observed in both silkworm and mulberry In order to get reliable information of genetic variation in molecular phylogenetic studies, at least 70 RAPD loci would be investigated And the results obtained in this study might be referential for AFLP and RFLP analysis