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OALib Journal期刊
ISSN: 2333-9721
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遗传  1998 

Differential Expression of the Gene in Early Somatic Embryogenesis of Lycium barbarum L by modified mRNA Differential Display
利用mRNA差别显示技术分析枸杞体细胞胚发生早期基因的差别表达 Differential Expression of the Gene in Early SomaticEmbryogenesis of Lycium barbarum L. by modified mRNA DifferentialDisplay

Keywords: mRNA,differential gene expression,PCR,somatic embryogenesis,Lycium barbarum L
mRNA,基因差别表达,PCR,体细胞胚发生,枸杞

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Abstract:

Embryogenic calli and early embryo can be obtained from both auxin and auxin-free medium The analysis of differential gene expression in early somatic embryogenesis has been hindered by above-mentioned material The modifications of the recently described mRNA differential display method were reported and differential gene expression in early somatic embryogenesis was analyzed We have obtained three differential bands of cDNA in early somatic embryogenesis The results indicate that gene expression has temperal and spalil order in early somatic embryogenesis of Lycium barbarum L Plant somatic embryogenesis is the results of differential gene expression in cell

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