Essential Role of Tetramethyl Ammonium Chloride for Amplification of Wheat Gene Sequences by Polymerase Chain Reaction(PCR)
四甲基氯化铵在PCR扩增小麦基因中的关键作用Essential Role of Tetramethyl Ammonium Chloride for Amplification of Wheat Gene Sequences by Polymerase Chain Reaction (PCR)

In the presence of tetramethyl ammonium chloride (TMACl), a chitinase gene sequence, a phenylalanine ammonia- lyase gene sequence and a glucanase cDNA sequence of wheat were amplified with highly degenerate primers by PCR. The inclusion of TMACl in the PCR reactions was essential for successful amplification of the desired sequences from genomic DNA or cDNA in wheat. The ends of the PCR fragments were made flush and phosphorylated prior to cloning. Sequence analyses of the above PCR fragments confirmed their identities, showing high sequence similarities to the genes used for the design of PCR primers.