A new method of microbial prospecting for oil and gas based on BIOLOG metabolic fingerprinting analysis
一种基于BIOLOG代谢指纹分析的油气微生物勘探新方法

Objective]Establishing a new method of microbial prospecting for oil and gas based on microbial community metabolic function.Methods]In this study,45 shallow reservoir soil samples and 25 non-reservoir soil samples were collected.Carbon catabolic activity of the microbial community was measured by biolog microplate,and microbial anomaly of reservoir was judged by discriminant function.Result] We screened 10 typical carbon sources of 95 carbon sources which could reflect the differences of reservoir and non-reservoir soil microbial community structure and made the validation test set to experimental zone and controlled zone samples using the discriminant function.The discriminate rate of reservoir and non-reservoir were 97.8% and 100% respectively and the overall accuracy was 98.6%.Conclusion] Biolog technology provided a highly efficient and precise method for preliminarily predicting oil and gas reservoir.