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OALib Journal期刊
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Application of Denaturing Gradient Gel Electrophoresis and Temperature Gradient Gel Electrophoresis in Microbial Molecular Ecology
DGGE/TGGE技术及其在微生物分子生态学中的应用

Keywords: DGGE,TGGE,Microbial diversity,Microbial molecular ecology
变性梯度凝胶电泳
,温度梯度凝胶电泳,微生物多样性,微生物分子生态学

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Abstract:

In recent years, denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. The technique is reliable, reproducible rapid and inexpensive. Here, the principle, the limitations and potentials application of DGGE/TGGE techniques are introduced in this paper.

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