Expression of PTX3 gene in Caco-2 cells treated with Lactobacillus acidophilus NCFM
嗜酸乳杆菌NCFM对Caco-2细胞中PTX3基因表达的影响

Objective]To study the expression of immunity and inflammatory mediator factor PTX3 in intestinal epithelial cells treated with Lactobacillus acidophilus NCFM and further to reveal the regulatory mechanism.Methods]Caco-2 cells were cocultured with Lactobacillus acidophilus NCFM for 0,2,4,8,12 h and 0,0.5,1,2,4 h respectively,then the total RNA and protein were extracted.The expression of PTX3 gene was analyzed by Real Time RT-PCR.The phosphorylation levels of NF-κB was analyzed by Western Blot.Caco-2 cells were pretreated with PDTC for 30 min before cocultured with Lactobacillus acidophilus NCFM for 2 h,then the total RNA was extracted and the expression of PTX3 gene was analyzed by Real Time RT-PCR.Results]Lactobacillus acidophilus NCFM could induce the expression of PTX3 in Caco-2 cells.The PTX3 expression peaked at 4 h after coculture.Then its expression gradually waned out.Lactobacillus acidophilus NCFM could rapidly activate the phosphorylation of NF-κB,and the expression of PTX3 was decreased notably after pretreated with PDTC for 30 min.Conclusion] Lactobacillus acidophilus NCFM could transiently regulate the immunity and inflammatory mediator factor PTX3 expression through rapidly activating NF-κB signaling pathway in Caco-2 cells.