Effect of Probiotics on Newcastle Disease Virus
几株益生菌的体外抗新城疫病毒作用

Abstract: Objective] To study the antiviral activity of probiotics and analyze its possible mechanism. Methods] We used the methods of NDV-HA and MTT to evaluate the NDV hemagglutination titer and inhibitory rate treated by probiotics in vitro and on CEF respectively. Results] Five probiotics and its metabolites could reduce the titer of NDV significantly, but the two pathogenic bacterium and their metabolites could not, these results indicated that probiotics may destruct the structure of NDV directly and this effect was strain-specific. Probiotics could increase the inhibitory rate of NDV significantly, and depended on concentration (P<0.01). The result of NDV inhibitory rate increased when the cells pre-treated with probiotics reflected that the probiotics may block NDV adsorbing to cells; from the phenomenon that probiotics and NDV added to cells at the same time reduced the CPE of cells, we could see that probiotics can destruct the structure of virus directly; the lowest inhibitory rate of NDV when cells infected with NDV before probiotics added showed that the probiotics will work hardly after the cells infected. Conclusion] Probiotics not only can destruct the virus, but also can block the virus infecting the cells and inhibit the proliferation of virus in cells.