Isolation,identification and enzyme profiling of an alkaliphilic protease producing bacterium
一株产蛋白酶嗜碱菌株的分离、鉴定及酶学特性

Objective] To isolate and characterize alkaliphilic bacteria with protease activity. Methods] Protease-producing alkaliphiles were isolated by skim milk agar method. The morphological,biochemical and physiological characteristics,16S rRNA gene sequence and DNA-DNA hybridization were determined to identify the taxonomic position of strain ZL223. Meanwhile,the enzyme characterization of the protease including optimal temperature and pH range,stability and oxidation resistance were tested by using casein hyd...