In vitro function of outer membrane protease T of Escherichia coli K1 pathogenic strain
大肠杆菌K1致病株外膜蛋白T体外功能

Plasminogen activation and antimicrobial peptide hydrolysis contribute to pathogens invasion and survival in vivo. Objective] To demonstrate the expression of outer membrane protease T in E. coli K1 pathogenic strain E44,its activity of plasminogen activator and protamine hydrolysis. Methods] After Benzamidine Sepharose Fast Flow and SOURCE 30Q chromatography,we got E44 outer membrane mixed fraction,and examined its activity of plasminogen activation with chromogenic substrate S-2251 method. An ompT delet...