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微生物学报 2010
The response of mouse macrophage cell line infected with BCG in vitro
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Abstract:
Objective]To illuminate the response of mouse macrophage cell line RAW264.7 after infected with Bacille Calmette-Guerin (BCG) in vitro. Methods] We analyzed the morphology and expression of co-stimulatory molecules on the surface of RAW264.7 after exposure to BCG for 23 hours. Then during the different culture time after discard the BCG in the supernatant, we analyzed the response of RAW264.7 by flow cytometry using carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE), annexin V/PI and Rhodamine 123...