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The response of mouse macrophage cell line infected with BCG in vitro
小鼠巨噬细胞系体外感染BCG的应答

Keywords: BCG,RAW264,7,apoptosis,co-stimulatory molecule,mitochondrial membrane potential
关键词:卡介苗,RAW264.7,细胞凋亡,共刺激分子,线粒体膜电位

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Abstract:

Objective]To illuminate the response of mouse macrophage cell line RAW264.7 after infected with Bacille Calmette-Guerin (BCG) in vitro. Methods] We analyzed the morphology and expression of co-stimulatory molecules on the surface of RAW264.7 after exposure to BCG for 23 hours. Then during the different culture time after discard the BCG in the supernatant, we analyzed the response of RAW264.7 by flow cytometry using carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE), annexin V/PI and Rhodamine 123...

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