Development of Genotyping System for Actinobacillus pleuropneumoniae and Its Clinical Application
胸膜肺炎放线杆菌基因分型方法的建立及其临床应用

To develop a PCR system bsaed on the genes of the three exotoxins(apx), outer membrane proteins(omlA), and transferring binding protein(tbpB) of Actinobacillus pleuropneumoniae. Founding that the resultant patterns of the reference strains for serovars 1,2,3,4,5,6,10 and 12 of biovar I of Actinobacillus pleuropneumoniae tested in this study to be distinguished. However, the reference strains for serovars7,8 and 15 could not be distinguished nor could the reference strains for serovars 9 and 11. The method can also be used to directly detect A. pleuropneumoniae infection in lung and tongsil tissue. The detection results of 42 tonsils and 126 lungs showed the potential clinicas application of the genotyping system.