Identification and Quantitation of Ceramides in Yeast Yarrowia lipolitica
解脂假丝酵母中神经酰胺的鉴别和定量分析

Lipids in yeast Yarrowia lipolitica was separated by preparative Thin Layer Chromatography (TLC) to obtain ceramide. Mass Spectrum (MS) and tandem-MS were applied to confirm the existence of the ceramide in Y. lipolitica and to reveal its structure. Combined with High Performance Liquid Chromatography and Evaporative Light Scattering Detector (HPLC/ELSD), for the first time, the ceramide content with 24 carbons in Fatty Acid (FA) side chain was determined in Y. lipolitica.