Recombinant cIL-18-binding protein as an antagonist to cIL-18 enhanced PBMCs secreting IFN-γ
重组IL-18BP拮抗重组鸡IL-18刺激外周血单核细胞产生IFN-γ的作用

Objective]Interleukin-18(IL-18) is a proinflammatory cytokines that plays a key role of immune regulation through activating the Th 1 cell and NK cell secreting interferon-γ(IFN-γ) .IL-18-binding proteins(IL-18BP) secreted in human and mouse can antagonise the activity of IL-18.The homologue gene of IL-18BP from fowlpox virus genome was predicted and identified of its expressed protein, to use as an antagonist to IL-18 guiding disease.Methods]The cIL-18BP gene was isolated from the genome of fowlpox vaccine virus by PCR through a pair of special primers and cloned into vectors pPICZαA, then expressed in yeast GS115.The biologic activity of recombinant proteins binding with cIL-18 was measured, inhibiting the activity of cIL-18 enhancing relative cells secreting IFN-γ was confirmed through ELISA.Results]The cIL-18BP gene was cloned from fowlpox virus and acquired high performance expression in yeast systems induced with methanol identified by SDS-PAGE.Recombinant cIL-18BP purified can bind specifically with recombinant cIL-18 determined by ELISA test.cIL-18BP can antagonise the activity of cIL-18 with determining the IFN-γ concentration secreting in PBMCs and MSB1 cells stimulated by cIL-18 respectively.Conclusion]The experiment indicates that recombinant cIL-18BP can inhibit the activity of cIL-18 stimulating related immune cells secreting IFN-γ.Deletion of the cIL-18BP from virus may improve the safety and immunogenicity of fowlpox virus vaccine.