Identification of the phaB genes and analysis of the PHBV precursor supplying pathway in Haloferax mediterranei
地中海富盐菌中phaB基因的鉴定及PHBV前体供应途径的分析

Objective] Identification and characterization of the genes involved in precursor supplying for poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV) biosynthesis in the haloarchaeon Haloferax mediterranei.Methods] By using BLAST(Basic Local Alignment Search Tool) search methodology,we obtained five genes(phaB1,phaB2,phaJ1,phaJ2 and phaJ3) that were possibly involved in the 3-hydroxyacyl-CoA precursor supplying for PHBV biosynthesis in H.mediterranei.Firstly,we proved that these five genes were all transcrib...