Expressing Antigen Region of VP2 Gene of Swine Vesicular Disease Virus in Escherichia coli
猪水泡病病毒VP2基因抗原区在大肠杆菌中的表达

The antigen region of VP2 gene of swine vesicular disease was amplified by RT-PCR and nPCR, the amplified fragments were cloned into the expression vector pGEX 4T-1.The insert position,the size and the reading frame were identified by PCR,restriction digestion and the sequence analysis of the recombinant plasmids. Results of SDS-PAGE indicated that the recombinant plasmids could express the VP2 gene of Swine vesicular disease. Western blot indicated that the positive serum of SVDV could react with expressed protein.