Effect of Sodium Citrate on The Growth Metabolism and Inosine Accumulation by Bacillus subtilis
柠檬酸钠对枯草杆菌生长代谢及肌苷积累的影响

The effects of sodium citrate on the growth metabolism and inosine accumulation by Bacillus subtillus were studied in this paper.When 0.2% sodium citrate was added into the basal medium,the inosine yield and the yield coefficient of inosine to glucose in the broth were increased 18% and 38% respectively.The analysis on the activities of some key enzymes during inosine fermentation revealed that the supplement of sodium citrate had profound effect on the activity of several key enzymes. The activities of Phosphofructokinase and Pyruvate Kinase were decreased remarkably,the flux of glycolysis pathway was thus cut down as well.