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OALib Journal期刊
ISSN: 2333-9721
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RAPD ANALYSIS OF THREE DIFFERENT COMPONENT NUCLEAR TYPES FROM FUSARIUM MONILIFORME HETEROKARYON TJ47-26
稻恶苗菌异核体三种核型核DNA的RAPD分析

Keywords: Fusarium moniliforme,Heterokaryon,RAPD
稻恶苗菌
,异核体,RAPD

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Abstract:

Random Amplified Polymorphic DNA(RAPD) technique was used to compare the nuclear DNA of different component nuclear types (homokaryons) of Fusarium moniliforme TJ47-26, which were designated as W(white), R(red) P(purple) respectively and derived from the one newly isolated heterokaryon. 80 random primers with 10 base pairs were used in RAPD experiments. Nearly 400 loci were detected and no polymorphic difference was found. The banding patterns of three different nuclear types were identical by using different primers. So it is deduced that the three different component nuclear types of F. moniliforme heterokaryon TJ47-26 probably came from the variation of the same nucleus.

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