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Classification for One Pathogenic Vibrio anguillarum Strain Isolated From Skin-ulcer Flounder
一株牙鲆皮肤溃烂症病原菌的鉴定

Keywords: Flounder,Vibrio anguillarum,16S rDNA,Phylogenetic analysis
牙鲆,
,鳗弧菌,,16S,rDNA,系统发育学分析

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Abstract:

A pathogenic bacterial strain M3 revealed gram negative rod shape, motile and translucent clone was studied. This bacterium was isolated from the skin-ulcer flounder in the fish farm of RongCheng City, Shandong Provice, and could not be identified by BIOLOG ID SYSTEM. Comparative 16S rDNA sequence analyses revealed that strain M3 was most related to the genus of Vibrio. The overall similarity value between strain M3 and Vibrio species were 94% to 98%. Phylogentic analysis showed that M3 strain exhibited the highest level of similarity to Vibrio anguillarum , and the biological features of strain M3 were very similar to that of Vibrio anguillarum. Based on the results obtained, strain M3 was identified as Vibrio anguillarum.

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