STUDY ON A NEW METHOD FOR FRANKIA IDENTIFICATION
一种弗兰克氏菌分种新方法的探讨

Amplification of the 16S-23 S rRNA intergenic spacer by polymerase chain reaction, with primers complementary to conserved motifs of 3'-end of 16S and 5'-end of 23S rRNA genes, revealed obvious size variation of this region among six Frankia strains. The PCR product of ArI4 was then analysed by gene cloning and DNA sequencing. The result of sequencing analysis showed this method to be a promising one for Frankia strains identification.