NUCLEOTIDE SEQUENCE OF gltB GENE ENCODING THE LARGE SUBUNIT OF RHODOBACTER SPHAEROIDES GLUTAMATE SYNTHASE
浑球红细菌谷氨酸合酶大亚单位基因(gltB)的序列分析

The complete nucleotide sequence of a 5.4-kb chromosomal EcoRI-Sall fragment was determined, which contains the structural gene (gltE) for the large subunit of Rhodobacter sphaeroides glutamate synthase, as well as the 5' - and 3'-flanking regions. A open reading frame of 4636 base pairs was identified as R. sphaeroides gltB gene- The MW of the large subunit, as deduced from the nucleotide sequence, was estimated as 164 kD. Comparision of the nucleotide sequences revealed a high similarity among gltB genes of R. sphaeroides, Azospirillum brasilense and Escherichia coll The deduced amino acid sequence of R. sphaeroides GltB showed a high similarity with that of A. brasilense GltB.