A SIMPLIFIED GRAM STAIN AND IT''S QUALITY CONTROL
革兰氏染色三步法与质量控制

A simplified Gram's staining method is described. The decolorization step of 95% alcohol in the original Gram's stain was omitted, and sub-stituted by a counter stain prepared with 0.4% fuchsin in alcohol which acts as both decolorization and counter staining in the simplified Gram staining method. Under quality control, a lot of different bacteria including Gram positive and negative organisms were stained with the simplified Gram staining method. The result was comparable to the original Gram stain. The simplified Gram staining method is a time-saving and practical method that could be used as a routine technic in the bacteriological laboratories.