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微生物学报 2001
THE CHARACTERIZATION OF pJW566 FROM L. LACTIS SUBSP. CREMORIS W56
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Abstract:
The plasmid pJW566 was isolated from L.lactis subsp. cremoris W56,one strain for Danish chadder mixed starter cultures.The strain containing plasmid pJW566 showed resistance against three common phages species 936,c2 and P335 worldwide.It was found that pJW566 encoded for an restriction and modification system, and showed strong resistance to phage CHCP412 when it was introduced into the industrial strain L.lactis CHCC2281 in milk medium.The endonuclease activity analysis indicated that the endonuclease required Mg 2 ,ATP, and was stimulated by AdoMet.
