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微生物学通报 2011
Comparison of pathogenic characteristics between PRRSV-GSLZ-1/2009 cell propagated virus and field sample virus
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Abstract:
The objective of this study was to analyze the correlation between genetic variation and pathogenicity of field sample virus during the propagation in sensitive cells. 36-day-old pigs were challenged with PRRSV GSLZ-1/2009 cell propagated virus and field sample virus after genetic variation analyse. Carry out real-time monitor and dynamics analyse to clinical symptoms, signs of change and the levels of antibody and inflammatory factors, and the histopathologic changes were observed under light microscope. The results showed that the disease-related genes was no variation, the antibody, inflammatory factors response and histopathologic lesions induced by field sample virus were more powerful than those induced by cell propagated virus. The researches indicated that virulence of field PRRSV could be weakened when it propagated in cells, and there are other possible factors except genetic variation playing on the role of virulence weaken.
