STUDY ON OPTIMIZATION OF GUANOSINE FERMENTATION
鸟苷发酵的优化研究

Bacillus subtilis AJ2066 was used to yield guanosine. The optimization of guanosine fermentation was studied by means of flask coupled with 50L auto-controlled fermentor. Monosodium glutamate is important to guanosine fermentation. The yield reached 31.49g/L in 50L fermentor with the medium supplemented with 1% monosodium glutamate. Hypoxanthine (Hx) can be used to synthesize guanosine directly as precursor. The yield reached 33.24g/L in 50L fermentor by supplementing 0.2% hypoxanthine during final stage of fermentation.