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微生物学通报 2010
Problems Analysis and Solution Strategies for Fluorescence Observation in VBNC Bacteria
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Abstract:
A detection method of viable but non-culturable bacteria by fluorescence microscopy was established with SYTO9 and PI staining cell nucleus, ink instead of Iraq black, specimens fixed by hand. Problems on background fluorescence, cell aggregation, blur fluorescence image, low micropore film absorbability, unclear bacterial form, and fluorescence quenching were analyzed. Some solution strategies were suggested in order to offer help for researchers.
