STUDY ON STABILITY OF DIAGONOSTIC TOOL ENZYMES UNDER TREHALOSE PROTECTION
海藻糖对医用诊断工具酶活性保护研究

The heat stability of three tool enzymes dried in trehalose at room temperature wasstudied for the possibility of preperation extraodinary stable enzyme reagent kit for serumcholesterol analysis. The cholesterol oxidase, cholesterol esterase and peroxidase have been driedin trehalose containing buffer and stored at 37c for 21O days, 55c for 15O days, 7Oc for4O days respectively. All of three enzymes achvity recovered more than 9O%. The trehaloseprotection is remarkably. In addition to concentration of trehalose, the buffer, ionic strength andpH are the main influence factors on stability of enzymes dried in trehalose.