Protecting Soft-shelled Turtle from Fatal Aeromonas Hydrophila Infection by Immunization with Aeromonas Hydrophila Emusion-oil Vaccine
嗜水气单胞菌佐剂灭活苗免疫中华鳖抵抗嗜水气单胞菌的致死性感染

We evaluated immune protection effectiveness of the aeromonas hydrophila emusion-oil vaccine in an immergence challenge model against haemorrhagic intestinal necrosis (HIN) and red abdominal shell by using soft-shelled turtles. In the active immune protection test, a high level of antibody was produced by the vaccine group. All the soft-shelled turtles in the vaccine group (10/10) were survived after challenged with 10 times 50% lethal dose (LD50) of virulent T3 strain, compared with 30% (3/10) survivors in the control group injected with 0.8% NaCl solution. In the passive immune protection test, 80% survivors (8/10) were observed in the group injected with serum, but only 20% (2/10) survivors were observed in the control group injected with 0.8% NaCl solution. These results showed that hydrophila Aeromonas emusion-oil vaccine had good immunogenicity, which could be used in the prevention of haemorrhagic intestinal necrosis (HIN) and red abdominal shell in soft-shelled turtles.