DEVELOPMENT OF AN INDUCIBLE EXPRESSION AND SECRETION SYSTEM FOR ALKALINE PROTEINASE IN BACILLUS SUBTILIS
枯草杆菌碱性蛋白酶基因诱导表达载体的构建

The regulatory region and the signal peptide sequence of the sacB gene has been amplified by PCR using Bacillus subtilis chromosomal DNA as template, and an inducible secretion vector has been developed based on this sequence, which was ligated with Bacillus subtilis alkaline proteinase gene. Transform Bacillus subtilis DB403 with this vector, and the expression of the inserted Bacillus subtilis alkaline proteinase gene can be induced by addition of sucrose into the medium.