PREPARATION OF MONOCLONAL ANTIBODIES (McAbs)AGAINST CANINE PARVOVIRUS AND COMPARISION BETWEEN MCABS AND SERA IN HEMAGGLUTINATION INHIBITION
犬细小病毒单克隆抗体的制备及其与免疫血清在血凝抑制中的比较

Spleen cells from a BALB/c mouse immunized with canine parvovirus (CPV) werefused with Sp2/0 myeloma cells by polyethylene glycol, After screening and cloning, 6 hybridcell lines steadily secreting monoclonal antibodies (McAb) were obtained. Hybridoma asciteswere used in hemagglutination inhibition (HI) test to detect samples known containing CPV,feline panleukopenie virus (FPLV),and mink enteritis virus (MEV) respectively, and fecalsamples from CPV infected dogs, compared with anti-CPV sera. The results suggested that HItest with CPV-McAbs was more specific and time-saving than that with anti-CPV sera, yet bothwere equally sensitive.