A STAlNING METHOD USED FOR OBSERVING PROTOPLASTSUNDER THE LIGHT MICROSCOPE
一种光学显微镜下观察原生质体的染色方法

Brevibacterium lactofermentum chltures digested by lysozyme were mixed with four dyes for microorganism staining, and then compared with observing effects of protoplasts under the light microscope. The results showed that the effect of the protoplasts was clearer, more convenient and better for photography in the specimens stained than that of unstained. The effects of ammonium oxalate-crystal violet and basic fuchsin dyes were better. This method has such superioties as simple procedure, convenient operation and obvious effect It can also be applied to observe the shape and the motion Pattern of the bacteria