THE STUDY ON DIFFERENTIATION AND DETECTION OFMYCOBACTERIUM SPECIES BY PCR AMPLIFICATION
应用PCR扩增对分枝杆菌分类鉴定及标本检测的研究

Using the prime b(special to M. tuberculosis) to amplify 21 mycobacterium and 13 nonmycobacterium, the products amplified were detected by agarose gel electrophoresis. The sensitivity was 50fg at annealing temperature 61 C ,and only M. tuberculosis, M. gastri were amplified, those results showed: the clinical isolated mycobacterium can be differentiated and detected quickly and efficiently by using the prime b(using the prime a when necessary) to amplify 16S～23S rDNA spacer sequence of mycobacterium.