STUDIES ON THE ADHERENCE PROPERTIES OF UROPATHOGENIC ESCHERICHIA COLI
致肾盂肾炎大肠杆菌粘附特性的研究

E. coli strains 132 and 136 isolated from urine of patients with pyelonephritis were sty-died on their adherence properties. The tested strains were found to agglutinate with the humanerythrocytes of P blood group and to attach to human urinary tract epithelial cells.The immuno-logical examination of the two strains was carried out with the antisera against the P pilus adhe-sive cluster of uropathogenic E. coli. The resuIts of their whole-bacterium ELISA showed to bepositive, and the antisera could be specifically combined with the P pili of E. coli 132 and 136 inthe immunogold electron microscopy. The crude pilus extracts of the two strains were preparedrespectively and tested by SDS-pAGEand western blotting. It was shown that the Mr of the Ppilus proteins from the tvvo clinical strains being 16.6Kd.Uropathogenic E. coli differs greatlyfrom other E. cofi strains in their adherence properties. So the above results revealed that E. coli132 and 136 were characterized as uropathogenic E. coli strains.